SURE : Shizuoka University REpository
نویسندگان
چکیده
23 In the present study, two LCYb genes (CitLCYb1 and CitLCYb2) were 24 isolated from Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus 25 sinensis Osbeck) and Lisbon lemon (Citrus limon Burm.f.) and their functions 26 were analyzed by the color complementation assay in lycopene-accumulating E. 27 coli cells. The results showed that CitLCYb1 and CitLCYb2 shared high identity 28 at the amino acid level among the three citrus varieties. The N-terminal region 29 of the two proteins encoded by CitLCYb1 and CitLCYb2 was predicted to 30 contain a 51-residue chloroplastic transit peptide, which shared low similarity. 31 In Satsuma mandarin, the secondary structures of the CitLCYb1 and CitLCYb2 32 encoding proteins without the transit peptide were quite similar. Moreover, 33 functional analysis showed that both enzymes of CitLCYb1 and CitLCYb2 34 participated in the formation of β-carotene, and when they were co-expressed 35 with CitLCYe, α-carotene could be produced from lycopene in E. coli cells. 36 However, although CitLCYb2 could convert lycopene to α-carotene in E. coli 37 cells, its extremely low level of expression indicated that CitLCYb2 did not 38 participate in the formation of α-carotene during the green stage in the flavedo. 39 In addition, the high expression levels of CitLCYb1 and CitLCYb2 during the 40 orange stage played an important role in the accumulation of β,β-xanthophylls in 41 citrus fruits. The results presented in this study might contribute to elucidate the 42 mechanism of carotenoid accumulation in citrus fruits.
منابع مشابه
Choreographic Three Bodies on the Lemniscate
Toshiaki Fujiwara†, Hiroshi Fukuda‡ and Hiroshi Ozaki¶ † Faculty of General Studies, Kitasato University, Kitasato 1-15-1, Sagamihara, Kanagawa 228-8555, Japan ‡ School of Administration and Informatics, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan ¶ Department of Physics, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan E-mail: † [email protected]...
متن کاملSeismic anisotropy of the uppermost mantle beneath the Rio Grande rift: Evidence from Kilbourne Hole peridotite xenoliths, New Mexico
a Graduate School of Science and Technology, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan b Géosciences Montpellier, Université Montpellier 2 and CNRS, Cc 060, Place E. Bataillon, 34095 Montpellier Cedex 5, France c Institute of Geosciences, Shizuoka University, Ohya 836, Shizuoka 422-8529, Japan d Department of Geological Sciences, University of Texas at El Paso, TX 79968, USA e Dep...
متن کاملIs the cosmological constant screened in Liouville gravity with matter?
Is the cosmological constant screened in Liouville gravity with matter? Takeo Inami1,2, Yoji Koyama3, Yu Nakayama4,∗, and Mariko Suzuki5,6 1Mathematical Physics Lab., Riken Nishina Center, Saitama 351-0198, Japan 2Department of Physics, National Taiwan University, Taipei 10617, Taiwan, R.O.C. 3Department of Physics, National Tsing-Hua University, Hsinchu 30013, Taiwan, R.O.C. 4California Instit...
متن کاملStudy of Surface Acoustic Wave Streaming Phenomenon Based on Temperature Measurement and Observation of Streaming in Liquids
Shihoko ITO, Mitsunori SUGIMOTO, Yoshikazu MATSUI, and Jun KONDOH Graduate School of Science and Engineering, Shizuoka University, Hamamatsu 432-8561, Japan Research Institute of Electronics, Shizuoka University, Hamamatsu 432-8561, Japan Department of Systems Engineering, Faculty of Engineering, Shizuoka University, Hamamatsu 432-8561, Japan Graduate School of Science and Technology, Hamamatsu...
متن کامل